Proteins tightly bound with rDNA transcribed regions in nuclei, nucleoids, and nucleoproteins of human lymphocytes: Isolation and characterization of candidate proteins

Autor:	Kovalev Li, D. M. Spitkovskii, Elizaveta S. Ershova, N. N. Veiko, N. A. Lyapunova
Rok vydání:	2000
Předmět:	Nuclease biology Biophysics Ribosomal RNA Molecular biology Nucleoprotein Affinity chromatography Structural Biology 28S ribosomal RNA parasitic diseases biology.protein Nucleoid Human genome Gene
Zdroj:	Molecular Biology. 34:250-255
ISSN:	1608-3245 0026-8933
DOI:	10.1007/bf02759647
Popis:	Proteins tightly bound with the transcribed regions of human ribosomal genes protect them fromRsaI nuclease upon digestion of isolated lymphocyte nuclei, nucleoids, and DNP. Three proteins (p43, p52, p67) specifically binding with region 9346–10,783 of the 28S rRNA gene were isolated by affinity chromatography. Complexes of rDNA with p52 and p67 were stable under high ionic strength. Only p52 was covalently bound with the 28S rRNA gene region in lymphocytes exposed in UV light.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3c65f6b50e441aaccdafa81c314d27b1 https://doi.org/10.1007/bf02759647 Zobrazit plný text záznamu Full text from SpringerLink