Thermostable inulin fructotransferase (DFA III-producing) from Arthrobacter sp. L68-1
| Autor: | Mitsuru Yoshida, Ken'ichi Ohtsubo, Kazutomo Haraguchi |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
Chromatography Polymers and Plastics biology Molecular mass Organic Chemistry Inulin Size-exclusion chromatography biology.organism_classification Enzyme assay chemistry.chemical_compound Enzyme chemistry Biochemistry Arthrobacter Materials Chemistry biology.protein Peptide sequence Bacteria |
| Zdroj: | Carbohydrate Polymers. 59:411-416 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2004.06.046 |
| Popis: | An inulin fructotransferase (DFA III-producing) [EC 2.4.1.93] from Arthrobacter sp. L68-1 was purified and characterised. The enzyme was purified from culture supernatant of the microorganism 54.2-fold with a yield of 16.0% using DEAE-Toyopearl chromatography (repeated twice) and Super Q-Toyopearl chromatography. The enzyme showed maximum activity at pH 5.5-6.0 and 55 °C. The enzyme activity was stable up to 80 °C after 1 h heat treatment. This heat stability was the highest of the inulin fructotransferases (DFA III-producing) reported to date. The molecular mass of the enzyme was estimated to be 43 kDa by SDS-PAGE and 73 kDa by gel filtration, and was considered to be a dimer. The N-terminal amino acid sequence (18 amino acid residues) was determined as AEETKGGPFNSPNAYDVT. |
| Databáze: | OpenAIRE |
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