Lupus Gut Microbiota Transplants Cause Autoimmunity and Inflammation
Autor: | Qiang Wei, Guobing Chen, Liangjing Lu, Lun He, Yiduo Sun, Yiyangzi Ma, Jiali Yuan, Xin Li, Mengtao Li, Zhao Li, Haitao Niu, Feng Gao, Gregg J. Silverman, Ruru Guo |
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Rok vydání: | 2021 |
Předmět: |
Systemic lupus erythematosus
biology business.industry Inflammation Gut flora biology.organism_classification medicine.disease_cause medicine.disease Autoimmunity Pathogenesis Immune system immune system diseases Immunology medicine biology.protein medicine.symptom Antibody skin and connective tissue diseases business Feces |
Popis: | BackgroundThe etiology of systemic lupus erythematosus (SLE) is multifactorial. Recently, growing evidence suggests that the microbiota plays a role in SLE, yet whether gut microbiota participates in the development of SLE remains largely unknown. To investigate this issue, we carried out 16s rDNA sequencing analyses in a cohort of 18 female un-treated active SLE patients and 7 female healthy controls, and performed fecal microbiota transplantation from patients and healthy controls to germ-free mice.ResultsCompared to the healthy controls, we found no significant different microbial diversity but some significantly different species in SLE patients including Turicibacter genus and other 5 species. Fecal transfer from SLE patients to germ free (GF) C57BL/6 mice caused GF mice to develop a series of lupus-like phenotyptic features, which including an increased serum autoimmune antibodies, and imbalanced cytokines, altered distribution of immune cells in mucosal and peripheral immune response, and upregulated expression of genes related to SLE in recipient mice that received SLE fecal microbiota transplantation (FMT). Moreover, the metabolism of histidine was significantly altered in GF mice treated with SLE patient feces, as compared to those which received healthy fecal transplants.ConclusionsOverall, our results describe a causal role of aberrant gut microbiota in contributing to the pathogenesis of SLE. The interplay of gut microbial and histidine metabolism may be one of the mechanisms intertwined with autoimmune activation in SLE. |
Databáze: | OpenAIRE |
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