Conformational changes induced in DNA by thein vitroreaction with the mutagenic amine: 3-N,N-acetoxyacetylainnio-4,6-dimethryldipyrido (l,2-a:3′,2′-d) imidazole

Autor:	Eric Hebert, Marc Leng, Bernard Loukakou, G. Saint-Ruf
Rok vydání:	1984
Předmět:	Circular dichroism Stereochemistry Guanine Biology Fluorescence In vitro Solvent chemistry.chemical_compound chemistry Biochemistry Genetics Imidazole Amine gas treating DNA
Zdroj:	Nucleic Acids Research. 12:8553-8566
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/12.22.8553
Popis:	The conformation of synthetic or natural DNAs modified in vitro by covalent binding of N-AcO-A-Glu-P-3 was investigated by fluorescence and circular dichroism. In all cases, substitution occurs mainly on the C8 of guanine residues. In modified poly(dG-dC).poly(dG-dC) or poly(dA-dC).poly(dG-dT) in B conformation, A-Glu-P-3 residues interact strongly with the bases whereas in Z conformation these residues are largely exposed to the solvent and interact weakly with the bases. A-Glu-P-3 and N-acetyl-2-aminofluorene (AAF) residues are equally efficient to induce the B-Z transition of poly(dG-dC).poly(dG-dC) and of poly(dA-dC).poly(dG-dT). Modifications of poly(dG).poly(dC) and calf thymus DNA indicate strong interactions between A-Glu-P-3 and the bases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3c57280bcf8736c5beafa60b72c35868 https://doi.org/10.1093/nar/12.22.8553 Zobrazit plný text záznamu