Changes in the blood plasma protein composition in an experiment with seven-day dry immersion

Autor: Oxana P. Trifonova, Irina M. Larina, I. I. Karuzina, M. A. Pyatnitskii, A. V. Lisitsa, L. Kh. Pastushkova, N. F. Samenkova
Rok vydání: 2012
Předmět:
Zdroj: Human Physiology. 38:772-775
ISSN: 1608-3164
0362-1197
DOI: 10.1134/s0362119712070237
Popis: This study was designed to track changes in the blood plasma proteome caused by exposure of healthy humans to dry immersion. The blood plasma specimens of five healthy volunteers aged 23–29 years obtained seven days before immersion, on day 7 of exposure in the immersion bath, and seven days after completion of the experiment were analyzed. After the extraction of major and the concentration of minor proteins, depleted blood plasma fractions were subjected to two-dimensional electrophoresis with the subsequent identification of significantly different protein spots with the method of mass-spectrometric analysis of peptide fragments. A significant change in intensity was observed for 25 protein spots with a coefficient of variation of >60%. Cluster analysis confirmed substantial changes in the blood plasma proteome in the period of readaptation of the body to the conditions of normal vital activity upon completion of immersion. On day 7 of readaptation, the apolipoprotein A-1, A-IV, and E concentrations were significantly higher compared to the baseline data and the measurements on day 7 of immersion. However, the levels of α-, β-fibrinogen, the complement C4B factor, and serum amyloid P were found to be lower.
Databáze: OpenAIRE
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