Comparison of direct immunofluorescence of exfoliated cells (DIF), tissue culture immunofluorescence (TCIF) and conventional virus isolation (CVI) for the diagnosis of respiratory virus infections

Autor:	Primrose Watkins, Claes Örvell, J.C. Booth, Shirlyn Grover
Rok vydání:	1990
Předmět:	Microbiology (medical) Paramyxoviridae biology medicine.diagnostic_test Viral culture viruses Immunology virus diseases biology.organism_classification Immunofluorescence Virology Virus Microbiology Measles virus Nasopharyngeal aspirate medicine Respiratory virus Direct fluorescent antibody
Zdroj:	Serodiagnosis and Immunotherapy in Infectious Disease. 4:59-66
ISSN:	0888-0786
DOI:	10.1016/0888-0786(90)90049-t
Popis:	Direct immunofluorescent staining (DIF) of exfoliated cells and immunofluorescent staining of infected tissue culture cells (TCIF) are superior to conventional virus isolation (CVI) techniques for the diagnosis of respiratory virus infections. Out of 197 specimens from patients with respiratory syncytial virus (RSV), influenza or parainfluenza virus, measles, adenovirus or cytomegalovirus (CMV) infections, DIF recognised 84%, TCIF 82% and CVI only 42%. DIF and TCIF were particularly useful for the diagnosis of RSV and parainfluenza virus infections, and the optimal specimen for diagnosis was nasopharyngeal aspirate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3c40aee92c5fe2dfca57cde8674654a8 https://doi.org/10.1016/0888-0786(90)90049-t Zobrazit plný text záznamu