Comparison of screening methods to evaluate the response of St. Augustine grass to Magnaporthe oryzae

Autor:	Ambika Chandra, A. D. Genovesi, C. F. Campos, S. P. Metz
Rok vydání:	2011
Předmět:	Veterinary medicine biology Stenotaphrum Stolon St. Augustine Grass food and beverages Plant Science Horticulture Plant disease resistance biology.organism_classification Plant disease Embryo rescue Genetic variation Botany Genetics Leaf spot Agronomy and Crop Science
Zdroj:	Plant Pathology. 61:255-261
ISSN:	0032-0862
DOI:	10.1111/j.1365-3059.2011.02521.x
Popis:	The objective of this study was to develop a reliable and high throughput screening method to evaluate the response of St. Augustine grass (Stenotaphrum secundatum) genotypes to the grey leaf spot (GLS) caused by Magnaporthe oryzae infection. Whole plant, detached stolon and detached leaf assays under growth chamber conditions were compared to field conditions on eight commercial and nine advanced breeding lines of St. Augustine grass. Disease was assessed using two variables, lesion size (LS) and overall plant disease severity (SEV). LS and SEV were highly correlated for field and growth chamber screening methods using the whole plant assay (LS r2 = 0·79; SEV r2 = 0·83; P ≤ 0·001), the detached stolon assay (LS r2 = 0·75; SEV r2 = 0·72; P ≤ 0·001), and the detached leaf assay (LS r2 = 0·46; SEV r2 = 0·60; P ≤ 0·001). Genotypic variation for resistance in 17 St. Augustine grass genotypes was identified using all screening methods for LS (P
Databáze:	OpenAIRE
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