Blueberry Supplementation Attenuates Inflammation and Oxidative Stress and Increases Brain‐Derived Neurotrophic Factor in the Brains of Middle‐Aged Mice Consuming a High Fat Diet
| Autor: | Abigail Rovnak, Barbara Shukitt-Hale, Amanda N. Carey, Kelsea R Gildawie, Casey K. Gilman |
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| Rok vydání: | 2015 |
| Předmět: |
Brain-derived neurotrophic factor
medicine.medical_specialty Calorie business.industry medicine.medical_treatment food and beverages Inflammation medicine.disease_cause Biochemistry Cytokine Endocrinology medicine.anatomical_structure Neurotrophic factors Cerebral cortex Internal medicine Genetics medicine Tumor necrosis factor alpha medicine.symptom business Molecular Biology Oxidative stress Biotechnology |
| Zdroj: | The FASEB Journal. 29 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Previously it was demonstrated that 9 month old mice fed a high fat diet (HFD, Harlan, TD.06414, 60% calories from lard fat) for five months had impaired memory; however supplementation of the HFD with 4% blueberry (BB, freeze-dried extract, U.S. Highbush Blueberry Council) reduced these memory deficits. The brains of these mice were dissected, homogenized, and assayed with ELISA for brain-derived neurotrophic factor (BDNF), the inflammatory cytokine tumor necrosis factor (TNF)-alpha, and were evaluated for oxidative stress by examining 4-Hydroxynonenal (4-HNE). There was a significant increase in hippocampal BDNF in the animals fed the HFD + BB (273±56 pg/ml/per mg of tissue) in comparison with the low fat diet (LFD, TD.08806, 10% calories from fat) and HFD-fed animals without BB supplementation (44±3 and 46±2 pg/ml, respectively). There was also a trend suggesting that animals fed a LFD + BB had elevated BDNF levels (101±57 pg/ml). Greater levels of TNF-alpha were found in the cerebral cortex of mice fe... |
| Databáze: | OpenAIRE |
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