| Autor: |
Kenzo Hiraoka, Hirokazu Hori, Lee Chuin Chen, Kunihiko Mori |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
International Journal of Mass Spectrometry. 279:41-46 |
| ISSN: |
1387-3806 |
| DOI: |
10.1016/j.ijms.2008.10.005 |
| Popis: |
The excitation of UV-absorbing MALDI matrixes with visible laser (532 nm wavelength) and the desorption/ionization of biomolecules were performed by coating the analytes doped matrix with Au thin film (5–10 nm) using ion sputtering deposition. The Au film was first ablated with the laser of higher fluence, resulting in a crater/hole about the size of the laser beam spot on the target. After a few initial laser shots, analytes and matrix related ions were observed from the crater even at lower laser fluence. Electron microscopy inspection on the laser ablated region revealed the formation of nanoparticles with sizes ranging from |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect