Formation of colonies of P-388 leukemic cells in semisolid agar

Autor:	Bibikova Mv, Ivanitskaia Lp, Navashin Sm, Egorov Lv
Rok vydání:	1978
Předmět:	Cloning Leukemia food.ingredient food medicine Agar General Medicine Biology medicine.disease Agar gel Suspension culture General Biochemistry Genetics and Molecular Biology Microbiology
Zdroj:	Bulletin of Experimental Biology and Medicine. 86:973-975
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf00801329
Popis:	By alternate passages of P-388 mouse ascites leukemia cells in a primary agarized culture and intraperitoneally in mice a substrain P-388/A2 adapted to culture in agar gel in the form of compact colonies without the use of special conditions of colony-stimulating factors was obtained. Reseeding efficiency and size of the colonies depended on the original density of the cell suspension. After the addition of 100 cells/ml seeding efficiency reached 20% and the number of cells in the colony by the 8th–10th day of culture was 13,000.
Databáze:	OpenAIRE
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