| Autor: |
Sotaro Shikano, Eriko Tsuboi, Kaori Soda, Ikuko Takeshita, Kenji Yamagishi, Yasuhiro Gon, Shu Hashimoto, Kazumichi Kuroda, Toshio Inoue, Shuichiro Maruoka |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
3.2 Airway Cell Biology and Immunopathology. |
| Popis: |
Background: Acute lung injury (ALI) is leading causes of mortality in critically ill patients. However, there is no useful biomarker that accurately reflects biological responses in the airway of ALI. Exosomes can serve as mediators of intercellular communication, such as messenger RNA (mRNA), microRNA and functional protein to neighboring cells. Objective: To determine whether lipopolysaccharide (LPS)-induced exosomal mRNAs in the bronchoalveolar lavage fluids (BALFs) accurately reflect inflammatory responses to LPS in the lung of animal model of ALI. Methods: We collected lung tissues and BALFs from LPS or PBS-exposed mice. We isolated total RNAs from lung tissues and examined global gene expression analysis using microarray. We also isolated exosomes from BALFs and quantified the number of exosomes using the q-Nano vesicle counter. Global mRNAs Expressions in exosomes were examined using next-generation sequencing techniques. Results: The number of exosomes in the BALFs of LPS-exposed mice did not show any change when compared to that of control mice. However, the total amount of RNA in exosomes significantly increased in the BALFs of LPS-exposed mice. We identified 67 LPS-inducible exosomal mRNAs in both the BALFs and lung tissues. After LPS-exposure, the change of expression levels between exosomal mRNA in the BALFs and mRNA in lung tissues were seen in direct correlation. Conclusion: Our data suggest that LPS-induced exosomal mRNAs in the BALFs accurately reflect biological response to LPS in the lung tissues. Thus, exosomal mRNAs in the BALFs might be useful biomarkers for ALI. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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