Thalidomide affects experimental endometriosis: A randomized controlled study in the rat

Autor: Afshin Azimirad, Zohreh Tavana, Saeed Alborzi, Perikala V. Kumar, Jaleh Zolghadri, Afsoon Zarei, Mina Azimirad
Rok vydání: 2014
Předmět:
Zdroj: Journal of Obstetrics and Gynaecology Research. 40:1989-1997
ISSN: 1341-8076
DOI: 10.1111/jog.12434
Popis: Aim This study aimed to assess the efficacy of thalidomide for treatment of experimental endometriosis. Methods This study was a parallel-group, double-blind, stratified, randomized controlled animal trial with 1:1 allocation ratio. Endometriosis was induced experimentally in 23 mature, nulligravid, female Sprague–Dawley rats, weighing approximately 200 g and aged 2 months. A checkpoint surgery was performed 6 weeks later. Then, the rats were randomly allocated into the thalidomide (22 mg/day p.o.) and control (0.5 mL saline 0.9%/day p.o.) groups of nine. After 6 weeks, they were killed. Before each laparotomy, blood for leukocyte and lymphocyte counts and during them, implants for histopathology and peritoneal fluid for interleukin (IL)-6, tumor necrosis factor-α and vascular endothelial growth factor (VEGF)-A concentrations (by enzyme-linked immunoassay) were collected. Allocation and stratified randomization were done using a computer, based on the obtained histopathology scores of the implants of the checkpoint surgery. Results The histopathology scores (the main outcome measure) were 2.00 ± 1.55 versus 0.44 ± 1.01 (P = 0.035). The comparisons of after-treatment counts of leukocytes, lymphocytes, VEGF-A and IL-6 between the two groups were statistically significant. Conclusion The results of this study are in favor of therapeutic implication of thalidomide in experimental endometriosis in rats. This is the first time thalidomide has been evaluated on endometriosis in an animal model.
Databáze: OpenAIRE
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