Inheritance of marker and target genes in seed and vegetative progenies of transgenic tobacco plants carrying the buckwheat serine protease inhibitor gene

Autor:	E. Yu. Yakovleva, N. V. Khadeeva
Rok vydání:	2010
Předmět:	Genetics biology Transgene food and beverages Kanamycin Genetically modified crops Plant disease resistance biology.organism_classification Genetic marker Plant virus medicine Cauliflower mosaic virus Gene medicine.drug
Zdroj:	Russian Journal of Genetics. 46:50-56
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795410010084
Popis:	The buckwheat serine protease inhibitor (BWI-1) target gene (ISP) was expressed under the control of the constitutive 35S promoter of the cauliflower mosaic virus was expressed in transgenic tobacco plants and conferred antibacterial resistance. A stable and linked inheritance and expression of the marker nptII and target genes were observed in a random sample of independent transgenic tobacco plants after longterm propagation by nodal segments or multiple (for 1.5 years) regenerations under nonselective conditions; the transgene insert was preserved in the T1 seed progeny. Transgenic plants displayed numerous alterations in microsporogenesis. A loss of kanamycin (Km) resistance was accompanied by a loss of antibacterial activity in two lines. Segregation was observed for Km resistance in line C7 and for seedling size in line C22.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3bd8ef2f73cdb338ac37712c43a9b4e4 https://doi.org/10.1134/s1022795410010084 Zobrazit plný text záznamu Full text from SpringerLink