Expression and functional analysis of an Entamoeba histolytica truncated adhesin in tomato plants

Autor:	Felipe Guadalupe Carrillo Landell, Teresa de Jesús Olivera Flores, Máximo B. Martínez, Guillermina García Rivera, Esther Orozco, Miguel Angel Gómez Lim
Rok vydání:	2008
Předmět:	Phagocytosis food and beverages Horticulture Biology biology.organism_classification medicine.disease Microbiology Bacterial adhesin Entamoeba histolytica Antigen Gene expression medicine Protozoa Amoebiasis Gene
Zdroj:	Plant Cell, Tissue and Organ Culture. 94:1-9
ISSN:	1573-5044 0167-6857
DOI:	10.1007/s11240-008-9379-8
Popis:	Several antigens from Entamoeba histolytica the agent causing human amoebiasis have been identified as potential vaccine candidates. One of them is the EhADH112 adhesin, which plays an important role during trophozoite adherence to and phagocytosis of target cells. We report here the expression of the EhADH112 carboxy-terminus of the gene (Adh240) in tomato plants. A 28 kDa band is recognized by specific antibodies in total proteins from transformed tomatoes. The plant-based adhesin conserves the ability to inhibit trophozoite adherence and phagocytosis of target cells by an average of 32.8 and 44.75% respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3bc1aa5c67320f5d7ce839755eab74c5 https://doi.org/10.1007/s11240-008-9379-8 Zobrazit plný text záznamu Full text from SpringerLink