Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis
| Autor: | Yueyun Hong, Ruth Welti, Xiangqing Pan, Mary R. Roth, Shivakumar P. Devaiah, Xuemin Wang |
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| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Phospholipase D media_common.quotation_subject Longevity food and beverages Cell Biology Plant Science Metabolism Biology biology.organism_classification Lipid oxidation chemistry Germination Arabidopsis Botany Genetics Food science Unsaturated fatty acid media_common Polyunsaturated fatty acid |
| Zdroj: | The Plant Journal. 50:950-957 |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/j.1365-313x.2007.03103.x |
| Popis: | Seed aging decreases the quality of seed and grain and results in agricultural and economic losses. Alterations that impair cellular structures and metabolism are implicated in seed deterioration, but the molecular and biochemical bases for seed aging are not well understood. Ablation of the gene for a membrane lipid-hydrolyzing phospholipase D (PLDalpha1) in Arabidopsis enhanced seed germination and oil stability after storage or exposure of seeds to adverse conditions. The PLDalpha1-deficient seeds exhibited a smaller loss of unsaturated fatty acids and lower accumulation of lipid peroxides than did wild-type seeds. However, PLDalpha1-knockdown seeds were more tolerant of aging than were PLDalpha1-knockout seeds. The results demonstrate the PLDalpha1 plays an important role in seed deterioration and aging in Arabidopsis. A high level of PLDalpha1 is detrimental to seed quality, and attenuation of PLDalpha1 expression has the potential to improve oil stability, seed quality and seed longevity. |
| Databáze: | OpenAIRE |
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