| Autor: | Yasushi Shiratori, Masao Omata, Masayuki Matsumura, Toru Nagura, Kazuo Kamii, Yohko Hikiba, Keiji Ohmura, Kenichi Okano |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
biology Lipopolysaccharide Physiology Kupffer cell Gastroenterology Stimulation Molecular biology Nitric oxide Nitric oxide synthase chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Hepatocyte Internal medicine medicine biology.protein Interferon gamma Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Digestive Diseases and Sciences. 43:1737-1745 |
| ISSN: | 0163-2116 |
| DOI: | 10.1023/a:1018879502520 |
| Popis: | To investigate the cellular communication in theliver, nitric oxide (NO) production by sinusoidal cellsand hepatocytes by stimulation with cytokines andKupffer cell-conditioned medium was quantitatively analyzed. NO production by the cells wasmeasured by the Griess reaction, and nitric oxidesynthase (iNOS) transcription level by a competitiveRT-PCR assay using mutant iNOS mRNA as a standard. NOproduction and iNOS mRNA transcriptional levels in Kupffercells were markedly increased by stimulation withlipopolysaccharide (LPS) and interferon-γ(IFN-γ), and moderately by interleukin-1β(IL-1β). NO production by hepatocytes was notsignificantly enhanced by LPS, but was markedly enhancedby IL-1β or the combination of tumor-necrosisfactor-α (TNF-α) and IFN-γ. HepatocyteNO production and iNOS mRNA levels were markedly enhanced bythe LPS-activated Kupffer cell conditioned medium, butthese effects were reduced by heat treatment or anti-TNFantibody. Although NG-monomethyl-L-arginine acetate and dexamethasone reduced NO productionby the cells, the iNOS mRNA level was reduced bydexamethasone only. Gel-shift assay showed NF-κBactivation in hepatocytes during this activation. These data reinforce the importance of cellularcommunication between sinusoidal cells andhepatocytes. |
| Databáze: | OpenAIRE |
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