| Popis: |
In order to demonstrate the usefulness of the “peptide segment separation method” for syntheses of large peptides and proteins, a model series of sequential polypeptides, Boc-[(Leu)3-(Pro)2-Gly]n-OBzl (n = 4, 6, 8, 10, and 12), were prepared by the reaction of Boc-[(Leu)3-(Pro)2-Gly]2-OH with H-[(Leu)3-(Pro)2-Gly]n-OBzl (n = 2, 4, 6, 8, and 10) using dicyclohexylcarbodimide and 1-hydroxy-1 H-benzotriazole as coupling reagents. The resulting peptides are assembled by the sequence of Gly-(Leu)3 separated by the (Pro)2 sequence. Regardless of the increase in peptide chain length of the amino component, the coupling reactions with 1-methyl-2-pyriolidone were achieved in high yields. Throughout the investigation, excellent solubility in usual polar solvents used for peptide syntheses is preserved. The purification of the peptide series by recrystallization could be completely achieved and HPLC on a gel filtration (styrenedivinylbenzene copolymer) column showed all the peptides to be monodisperse. |
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