Synergistic activity of dobutamine combined with azoles and its mechanism of action against strains ofCandida glabrata
| Autor: | Fatima DD Barroso, Lisandra J da Silva, Lívia GAV Sá, Cecília R da Silva, João BA Neto, Francisca BA do Nascimento, Helaine A Queiroz, Amanda C Leitão, Vitória PF Cabral, Daniel S Rodrigues, Amanda D Barbosa, Bruno C Cavalcanti, Manoel O Morais, Hélio VN Júnior |
|---|---|
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Future Microbiology. 17:437-448 |
| ISSN: | 1746-0921 1746-0913 |
| DOI: | 10.2217/fmb-2020-0256 |
| Popis: | Aims: To evaluate the antifungal effect of dobutamine against Candida glabrata as well as its synergism with azoles and its action on biofilm. Methods: The M27-A3 protocol and flow cytometry were used for elucidation of the possible mechanism of action. Results: The tested isolates presented MICs ranging from 2 to 32 μg/ml for dobutamine, with fungistatic effect. A total of 82% of the strains showed synergism with fluconazole, with 90% showing synergism with itraconazole. The effect on biofilm formation was nonsignificant. Cytometry tests showed that dobutamine induced mitochondrial depolarization. Conclusion: Dobutamine has an antifungal effect on strains of C. glabrata and synergistic activity with azoles. This effect is probably mediated by increased oxidative damage to the membrane. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|