Specific expression of olfactory binding protein in the aerial olfactory cavity of adult and developing Xenopus

Autor:	Claire Fenech, Jean-Claude Pernollet, Julie Millery, Jean Gascuel, Loïc Briand, Florence Blon, Brigitte Quennedey, Valérie Bézirard, Laurent Richard-Parpaillon
Rok vydání:	2005
Předmět:	Olfactory system 0303 health sciences Messenger RNA biology General Neuroscience media_common.quotation_subject Binding protein fungi Xenopus food and beverages Olfaction Anatomy biology.organism_classification DNA-binding protein Cell biology 03 medical and health sciences 0302 clinical medicine Odorant-binding protein biology.protein Metamorphosis 030217 neurology & neurosurgery 030304 developmental biology media_common
Zdroj:	European Journal of Neuroscience. 22:1389-1399
ISSN:	1460-9568 0953-816X
DOI:	10.1111/j.1460-9568.2005.04337.x
Popis:	Olfactory binding proteins (OBP), commonly associated with aerial olfaction, are found in the olfactory mucus of mammals but have never been identified in fish. It is still not clear whether the presence of OBP in aerial olfactory systems is due to phylogenetic or to functional differences linked to the adaptation of the olfactory system to an aerial environment. To test this alternative, the olfactory system of Xenopus offers a unique opportunity because it includes two olfactory cavities, one of which is thought to be devoted to aquatic olfaction and the other to aerial olfaction. We therefore purified and cloned OBPs in two Xenopus species. Xenopus laevis OBP (XlaeOBP) and Xenopus tropicalis OBP (XtroOBP) exhibit 158 and 160 amino acids, respectively, sharing 89 residues. cRNA probes allowed us to demonstrate that XlaeOBP and XtroOBP are expressed at the level of Bowman's gland specifically in the aerial olfactory cavity, as confirmed using anti-XlaeOBP antiserum. OBP mRNA transcription occurs early during metamorphosis, as early as stage 57. This is the first study to demonstrate that OBPs are exclusively present in the aerial chamber and are only expressed as the tadpole becomes an adult in species which possess both aquatic and aerial olfactory organs.
Databáze:	OpenAIRE
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