Identification and characterization of a sulfite reductase gene and new insights regarding the sulfur-containing amino acid metabolism in the basidiomycetous yeast Cryptococcus neoformans
| Autor: | Kiminori Shimizu, Akio Toh-e, Katsuhiko Kamei, Phuong Thao Nguyen, Akira Watanabe, Yumi Imanishi-Shimizu, Ngoc Hung Nguyen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Cryptococcus neoformans 0303 health sciences Methionine 030302 biochemistry & molecular biology Antifungal drug Sulfurtransferase General Medicine Biology biology.organism_classification Sulfite reductase Amino acid 03 medical and health sciences chemistry.chemical_compound Metabolic pathway chemistry Biochemistry Genetics Amino acid synthesis 030304 developmental biology |
| Zdroj: | Current Genetics. 67:115-128 |
| ISSN: | 1432-0983 0172-8083 |
| Popis: | The amino acid biosynthetic pathway of invasive pathogenic fungi has been studied as a potential antifungal drug target. Studies of the disruption of genes involved in amino acid biosynthesis have demonstrated the importance of this pathway in the virulence of Cryptococcus neoformans. Here, we identified the MET5 (CNL05500) and MET10 (CNG03990) genes in this pathway, both encoding sulfite reductase, which catalyzes the reduction of sulfite to sulfide. The MET14 (CNE03880) gene was also identified, which is responsible for the conversion of sulfate to sulfite. The use of cysteine as a sulfur source led to the production of methionine via hydrogen sulfide synthesis mediated by CYS4 (CNA06170), CYS3 (CNN01730), and MST1 (CND03690). MST1 exhibited high homology with the TUM1 gene of Saccharomyces cerevisiae, which has functional similarity with the 3-mercaptopyruvate sulfurtransferase (3-MST) gene in humans. Although the hypothesis that hydrogen sulfide is produced from cysteine via CYS4, CYS3, and MST1 warrants further study, the new insight into the metabolic pathway of sulfur-containing amino acids in C. neoformans provided here indicates the usefulness of this system in the development of screening tools for antifungal drug agents. |
| Databáze: | OpenAIRE |
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