Vitexin alleviates neuropathic pain in a mouse chronic constriction injury model by inactivation of NF-κB
| Autor: | Liangyu Sheng, Gonghao Zhan, Wentong Xu, Yanwei Chen, Xueli Zhu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.diagnostic_test
business.industry Therapeutic effect Vitexin Pharmaceutical Science NF-κB Pharmacology nervous system diseases chemistry.chemical_compound Western blot chemistry Neuropathic pain Hyperalgesia medicine Pharmacology (medical) Tumor necrosis factor alpha Sciatic nerve medicine.symptom business |
| Zdroj: | Tropical Journal of Pharmaceutical Research. 19:989-994 |
| ISSN: | 1596-9827 1596-5996 |
| DOI: | 10.4314/tjpr.v19i5.12 |
| Popis: | Purpose: To investigate the therapeutic effect of vitexin on neuropathic pain (NP) in a mouse model of chronic constriction injury (CCI).Methods: The CCI model was established by four chronic ligatures in the sciatic nerve. Vitexin was intraperitoneally administered (10 mg/kg, once daily) for 21 days. Mechanical withdrawal threshold (MWT) and paw withdrawal latency (PWL) were determined before and after the establishment of CCI model. The spinal cords were collected to measure mRNA levels by reverse-transcriptase polymerase chain reaction (RT-PCR) enzyme-linked immunosorbent assay (ELISA). Western blot was used to examine protein expression levels.Results: Vitexin reversed the CCI-induced reduction in MWT and PWL values, indicating that it lowered mechanical hypersensitivity response and hyperalgesia caused thermal stimulation (p < 0.05). The elevated levels of IL-6, IL-1β, and TNFα observed in CCI-treated mice were also inhibited by vitexin, suggesting that it suppressed pro-inflammatory cytokines. Moreover, vitexin attenuated CCI-induced activation of NF-κB signaling in CCI-treated mice (p < 0.05).Conclusion: Vitexin alleviates NP by inhibiting pro-inflammatory cytokines and NF-κB signaling in CCItreated mice. Thus, it is a potential target for NP treatment. Keywords: Vitexin, Neuropathic pain, Chronic constriction injury, Mechanical hypersensitivity, Hyperalgesia, NF-κB |
| Databáze: | OpenAIRE |
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