Localization of actin and characterization of its isoforms in the hyphae ofNeurospora crassa

Autor:	Bich-Nga Nguyen Thi, Gilbert Turian, Francisco Barja
Rok vydání:	1991
Předmět:	Gel electrophoresis biology Phalloidin fungi Arp2/3 complex macromolecular substances Microfilament biology.organism_classification Microbiology Neurospora crassa chemistry.chemical_compound chemistry Biochemistry Organelle Genetics biology.protein Deoxyribonuclease I Molecular Biology Actin
Zdroj:	FEMS Microbiology Letters. 77:19-24
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1991.tb04315.x
Popis:	The actin of Neurospora crassa wild type Strain St. Lawrence has been purified, characterized and localized. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-Sepharose. This protein was identified as actin on immunoblots when an anti-actin monoclonal antibody raised against chicken gizzards was used as a probe. After two-dimensional gel electrophoresis three actin isoforms were detected. The distribution of actin in hyphae was examined by FITC-phalloidin staining of formaldehyde fixed hyphae. F-actin was found to be mainly concentrated in the hyphal tips in which it formed a uniform cap. Apical actin could be involved in hyphal morphogenesis, organelle motility and maintenance of polarity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3b3226cfa667ab33d9b045e9b9d8fdbc https://doi.org/10.1111/j.1574-6968.1991.tb04315.x Zobrazit plný text záznamu Plný text