| Autor: |
C. Kleuss |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Ciba Foundation Symposium 190-Somatostatin and its Receptors |
| DOI: |
10.1002/9780470514733.ch11 |
| Popis: |
In rat pituitary GH3 cells Ca2+ current through L-type channels is reduced by somatostatin. This modulation of channel activity by somatostatin receptors is mediated by a guanine nucleotide-binding regulatory protein (G protein). It is sensitive to pertussis toxin, indicating the involvement of a G(o)- or Gi-type G protein in this pathway. The identity of this G protein was determined by suppressing the expression of endogenous G proteins individually via intranuclear injection of antisense oligonucleotides. This method was applied to GH3 cells to screen several G protein alpha, beta and gamma subunits for their roles in the defined signal transduction pathway. The loss of somatostatin's modulating activity on the voltage-dependent Ca2+ channel after oligonucleotide injection revealed the involvement of G(o) alpha 2 beta 1 gamma 3 to the exclusion of other closely related subtypes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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