Interleukin-1β Induces Interleukin-lα Messenger Ribonucleic Acid Expression in Primary Cultures of Leydig Cells*

Autor:	Tu Lin, Weiwei Chang, J. Harrington Calkins, Madan L. Nagpal, Deli Wang, M. Michael Sigel
Rok vydání:	1991
Předmět:	Messenger RNA medicine.medical_specialty Leydig cell medicine.medical_treatment Interleukin Biology Paracrine signalling Endocrinology medicine.anatomical_structure Cytokine Cell culture Internal medicine Gene expression medicine Autocrine signalling
Zdroj:	Endocrinology. 129:2862-2866
ISSN:	1945-7170 0013-7227
Popis:	Previously, we have reported that interleukin-1 (IL-1) can modulate Leydig cell steroidogenesis. Recently, IL-1-like material has been shown to be present in the testis; however, the cellular source of this material remains unclear. In the present study we found that human recombinant IL-1β (1–100 ml/ng) caused dose-dependent increases in IL-1α mRNA expression in Leydig cells. Similar to that reported in other tissues, IL-1α mRNA from Leydig cells is mainly 2.2 kilobases. IL-1α mRNA expression in Leydig cells was detectable as early as 2 h after the addition of IL-1β (10 ng/ml) and persisted for up to 24 h. Lipopolysaccharide also stimulated IL-1α mRNA expression in these cells, but phorbol ester had no effect. Our results indicate that Leydig cells are a potential source of IL-1, which has both autocrine and paracrine effects. (Endocrinology 129: 2862–2866, 1991)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3b244c3ca77323a11a5d96c91ced141f https://doi.org/10.1210/endo-129-6-2862 Zobrazit plný text záznamu