Fish erythrocytes as a tool to study temperature-induced responses in plasma membranes

Autor: T. Szegletes, J. Nemcsok, Indranil Dey, Tibor Farkas, Cs. Buda
Rok vydání: 1993
Předmět:
Zdroj: Lipids. 28:743-746
ISSN: 1558-9307
0024-4201
DOI: 10.1007/bf02535997
Popis: The dorsal aorta of carp (Cyprinus carpio L.) was cannulated, and the fish were kept in thermostated aquaria at 5°C for 24h. The water temperature was then gradually increased to 25°C at a rate of 0.5°C/h, and then decreased to 5°C at the same rate. Blood was withdrawn at five-degree intervals to determine the fluidity of erythrocyte plasma membranes uponex vivo incorporation of the fluorescent dye, 3-[p-(6-phenyl-1,3,5-hexatrienyl)phenyl] propionic acid. Steady-state anisotropy (Rss) of the plasma membranes increased and decreased with the increase and decrease in water temperature, respectively. The sterol-to-phospholipid ratio remained unchanged throughout the thermal shifts. The fatty acid compositions of the total phospholipids, of phosphatidylcholine and of phosphatidylethanolamine remained virtually unchanged, except for the level of arachidonic acid, which increased in erythrocytes from fish at the higher temperature (25°C). The molecular species compositions of phosphatidylcholines and phosphatidylethanolamines also remained unaffected throughout the thermal shifts. The erythrocyte plasma membranes were more responsive to temperature shiftsin vivo thanin vitro when percent efficacy was compared. Thus, factors other than lipid changes are conceivably involved in the adaptation of erythrocyte plasma membranes to short-term thermal changes.
Databáze: OpenAIRE
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