Abstract P057: Adipocyte Progenitor Cells In Perivascular Adipose Tissues: The Effect Of Sex And Anatomical Location

Autor: Cristian J Rendon Mora, Emma D Flood, Stephanie W Watts, G A Contreras
Rok vydání: 2022
Předmět:
Zdroj: Hypertension. 79
ISSN: 1524-4563
0194-911X
DOI: 10.1161/hyp.79.suppl_1.p057
Popis: Perivascular adipose tissue (PVAT) modifies vascular function due to its capacity to synthesize vasoactive products and its mechanical properties. During cardiovascular diseases (CVD), changes in adipocyte populations affect PVAT function. In obesity, expansion of adipose tissue (AT) differs between sex in rodents and humans in a site-dependent manner. Consequently, men are more susceptible to obesity-associated hypertension. However, how adipocyte progenitors (APC) contribute to those differences in PVAT depots is currently unknown. Our objective was to evaluate the distribution of APCs among different AT sites in both sexes under normotensive conditions using a genetic lineage tracing mouse model. We hypothesize that APC population distribution is affected by sex and anatomical location. PVAT from abdominal (abPVAT) and thoracic (atPVAT) aorta, mesenteric arteries (mesPVAT), and non-PVAT subscapular (BAT), perigonadal (GON), and subcutaneous (SCAT) were collected from 30-week-old female and male double transgenic mice ( PDGFRa -CreER T2 /R26-LSL-tdTomato; n=13). In this model, tamoxifen administration (5d, 150 mg/kg) induces PDGFRa + cells well-defined APC populations, to express the fluorescent tdTomato reporter. To harvest APC, AT was minced, digested [Liberase™ (50μg/ml)] for 1hr at 37°C, and then filtered with a 40μm cell strainer. The cell suspension was incubated with conjugated antibodies anti CD45, and CD31. Zombie NIR was used to exclude dead cells. Next, flow cytometry was performed to determine APC population distribution defined as CD45 - , CD31 - , and tdTomato + ; results are shown as % APC±SEM. PDGFRα + are present in all AT tissues. Among PVAT sites, abPVAT had fewer APC (1.17±0.25) compared with GON (5.6±0.25; P
Databáze: OpenAIRE
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