Mesenchymal stem cell interacted with PLCL braided scaffold coated with poly-l -lysine/hyaluronic acid for ligament tissue engineering
| Autor: | Ghislaine Cauchois, Cédric Laurent, Yun Chen, Natalia de Isla, Laurie Targa, Xiong Wang, Xing Liu, Qiaoyue Du |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold Materials science Biocompatibility Mesenchymal stem cell Metals and Alloys Biomedical Engineering 02 engineering and technology 021001 nanoscience & nanotechnology Biomaterials 03 medical and health sciences chemistry.chemical_compound Collagen Type III 030104 developmental biology medicine.anatomical_structure chemistry Tissue engineering Hyaluronic acid Ceramics and Composites Ligament medicine Fiber 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. 106:3042-3052 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.36494 |
| Popis: | The challenge of finding an adapted scaffold for ligament tissue engineering remains unsolved after years of researches. A technology to fabricate a multilayer braided scaffold with flexible and elastic poly (l-lactide-co-caprolactone) (PLCL 85/15) has been recently pioneered by our team. In this study, polyelectrolyte multilayer films (PEM) with poly-l-lysine (PLL)/ hyaluronic acid (HA) were deposited on this scaffold. After PEM modification, polygonal (PLL) and particle-like (HA) structures were present on the braided scaffold with no significant variation of fibers Young's modulus. Wharton's jelly mesenchymal stem cells (WJ-MSC) and bone marrow mesenchymal stem cells (BM-MSC) showed good metabolic activity on scaffolds. They presented a spindled shape along the fiber longitudinal direction, and crossed the fibers to form cell bridges. Collagen type I, collagen type III, and tenascin-C secreted by MSCs were detected on day 14. Moreover, one-layer modified scaffold presented increased chemotaxis. As a conclusion, our results indicate that this braided PLCL scaffold with one-layer PEM modification shows inspiring potential with satisfying mechanical properties and biocompatibility. It opens new perspectives to incorporate growth factors within PEM-modified braided PLCL scaffold for ligament tissue engineering and to recruit endogenous cells after implantation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3042-3052, 2018. |
| Databáze: | OpenAIRE |
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