Synthesis of 1-O-[hexadecyl-1′,2′-3H]hexadecyl 2-acetyl-sn-glyceryl 3-phosphorylcholine and 1-O-alkyl [32P]lysophosphatidycholine
| Autor: | Puliyur Srinivasan, Yang Hong, Un Hoi Do, Peter Tam |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Phosphorylcholine Organic Chemistry Phospholipid Cytidine Ether Alkaline hydrolysis (body disposal) Biochemistry Medicinal chemistry Analytical Chemistry chemistry.chemical_compound Lysophosphatidylcholine chemistry Phosphatidylcholine Drug Discovery Organic chemistry lipids (amino acids peptides and proteins) Radiology Nuclear Medicine and imaging Spectroscopy Alkyl |
| Zdroj: | Journal of Labelled Compounds and Radiopharmaceuticals. 38:117-127 |
| ISSN: | 0362-4803 |
| DOI: | 10.1002/(sici)1099-1344(199602)38:2<117::aid-jlcr829>3.0.co;2-4 |
| Popis: | 1-O-[hexadecyl-1',2'-3H]Hexadecyl 2-acetyl-sn-glyceryl 3-phosphoryl-choline (GPC) was prepared by acetylation of 1-O-alkenyl lysophosphatidylcholine, reduction of the alkenyl lipid with tritium gas over palladium oxide, and separation of molecular species of 1-O-[ 3 H]alkyl 2-acetyl GPC by reverse phase thin-layer chromatography. 1-O-Alkyl [32P]lysophosphatidylcholine was prepared by mild alkaline hydrolysis of 1-O-alkyl 2-acyl [32P]phosphatidylcholine, which was enzymatically produced by treating cytidine 5'-[ 32 P]diphosphocholine with 1-O-hexadecyl 2-acetyl-sn-glycerol in the presence of rat liver microsomes. The resulting radiolabeled ether phospholipids possessed high specific radioactivity suitable for metabolic and binding studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text