| Autor: |
Tony George Jacob, Tara Sankar Roy, Shubhi Saini, D.N. Bhardwaj |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Journal of the Anatomical Society of India. 64:99-106 |
| ISSN: |
0003-2778 |
| DOI: |
10.1016/j.jasi.2015.10.011 |
| Popis: |
Introduction Age associated progressive fibrosis may be a major causative factor that leads to pathogenesis of many diseases. Activated pancreatic stellate cells (α-SMA positive) play a major role in fibrogenesis that affects the cytoarchitecture and functioning of pancreas. This study dealt with age-related fibrotic changes in the ductular system of the tail and body of pancreas and the morphology of pancreatic stellate cells. Methods Pancreata ( n = 36) from cadavers aged 30–80 years were obtained after due clearances and processed for Masson's trichome staining. Fibrosis was quantified using Adobe Photoshop (CS2) and Image-J software. Hierarchical cluster analysis was done on the luminal area and total ductal area that were measured by the nucleator probe of StereoInvestigator software (MBF, Vermont, USA). Pancreatic stellate cells (α-SMA positive cells) were identified by immunohistochemistry and quantified stereologically around periacinar, periductular, perivascular, and peri-Islet areas. Results An increased fibrosis was noted in body and tail regions of the pancreas with increasing age. Three duct populations were identified in clustering. Their area and corresponding lumen showed a significant increase with progressive decades ( p p = 0.002, 0.004 and 0.002, respectively). Discussion Pancreatic stellate cells may be important contributors to increased fibrosis in pancreas. The classification of pancreatic ducts into three clusters may serve to be a useful tool. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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