Comparison of newly developed hydroxyl-functionalized monodisperse HILIC columns new HILIC column
| Autor: | Ali Tuncel, Saliha Dinç, Saba Samatya Olmez |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Diethanolamine
Chromatography 010405 organic chemistry Ethylene glycol dimethacrylate Hydrophilic interaction chromatography 010401 analytical chemistry Clinical Biochemistry Pharmaceutical Science Methacrylate 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Ethanolamine chemistry Polymerization Triethanolamine medicine Derivatization medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 40:649-655 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1080/10826076.2017.1343731 |
| Popis: | In this study, new hydroxyl-functionalized monodisperse polymeric hydrophilic interaction chromatography (HILIC) columns were developed using different derivatization agents. In addition, the influences of derivatization temperature of the best agent and polymer composition on the separation were investigated under HILIC conditions. Monodisperse–porous hydrophilic particles were synthesized by the seeded polymerization method using 3-chloro-2-hydroxypropile methacrylate (HPMA-CL) and ethylene glycol dimethacrylate (EGDMA) monomers. The chloropropyl terminal ends of the poly(HPMA-Cl-co-EGDMA) particles were derivatized with amine group of ethanolamine (EA), diethanolamine, and triethanolamine (TEA) at 80°C through nucleophilic reaction. The performance of synthesized particles was evaluated with the amount of ligand on the particle surfaces, column backpressure, and separation power under HILIC condition. TEA was found to be the best derivatization agent for the separation of toluene, acrylamide, t... |
| Databáze: | OpenAIRE |
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