Investigation of potential degradation products of a newly synthesised β-lactam antibiotic by multi-stage liquid chromatography–electrospray mass spectrometry
| Autor: | Andrea Casazza, Marco Garzotti, Luca Rovatti, Mahmoud Hamdan |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Electrospray
Chromatography Chemistry Ammonium phosphate Organic Chemistry General Medicine Mass spectrometry Biochemistry High-performance liquid chromatography Sample preparation in mass spectrometry Analytical Chemistry chemistry.chemical_compound Liquid chromatography–mass spectrometry Bromide Antibacterial agent |
| Zdroj: | Journal of Chromatography A. 819:133-142 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(98)00485-3 |
| Popis: | This work describes the use of multi-stage liquid chromatography–electrospray mass spectrometry to characterise four potential degradation products associated with a suspension containing a newly synthesised β-lactam antibacterial compound. Initial identification of these degradates was obtained by analytical LC in which the mobile phase contained cetyltrimethylammonium bromide as ion-pairing agent and ammonium phosphate buffer, both of which are highly involatile making them incompatible with electrospray mass spectrometry. The use of multi-stage liquid chromatography maintained the initial LC conditions necessary for the desired separation and at the same time facilitated the use of mass spectrometry detection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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