Structural Study of H,K-ATPase by Electron Microscopy and Image Reconstruction

Autor:	Manijeh Mohraz, Sonal Sathe, P.R. Smith
Rok vydání:	1990
Předmět:	Nuclear magnetic resonance biology law Chemistry ATPase biology.protein General Medicine Iterative reconstruction Electron microscope law.invention
Zdroj:	Proceedings, annual meeting, Electron Microscopy Society of America. 48:94-95
ISSN:	2690-1315 0424-8201
DOI:	10.1017/s0424820100179221
Popis:	H,K-ATPase is an integral protein of the plasma membrane of parietal cells. It is believed to constitute the pump responsible for secretion of acid into stomach. Its catalytic subunit (Mr 110,000) shows striking sequence homology to those of other transport ATPases. Recent studies suggest that there is also a glycoprotein (ca 300 amino acids) associated with the H,K pump, which is very homologous to the β subunit of the Na,K-ATPase.The enzyme is isolated in protein-rich membrane vesicles from hog stomachs. Formation of two-dimensional crystals is induced in suspensions of the enzyme by methods that had proved successful for crystallization of the Na,K-ATPase.
Databáze:	OpenAIRE
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