Elevated CD14 (Cluster of Differentiation 14) and Toll-Like Receptor (TLR) 4 Signaling Deteriorate Periapical Inflammation in TLR2 Deficient Mice
Autor: | Winston Patrick Kuo, Laila A. Bahammam, Shuang Xu, Philip Stashenko, Alexander J. Trachtenberg, Kimito Hirai, Hajime Sasaki, Daniel B. Rider, Mako Susa, Akira Fujimura, Hisako Furusho |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Toll-like receptor Histology Cluster of differentiation business.industry Inflammation 030206 dentistry Proinflammatory cytokine 03 medical and health sciences TLR2 030104 developmental biology 0302 clinical medicine Immunology TLR4 medicine Anatomy medicine.symptom Signal transduction Receptor business Ecology Evolution Behavior and Systematics Biotechnology |
Zdroj: | The Anatomical Record. 299:1281-1292 |
ISSN: | 1932-8486 |
DOI: | 10.1002/ar.23383 |
Popis: | Apical periodontitis (periapical lesions) is an infection-induced chronic inflammation in the jaw, ultimately resulting in the destruction of apical periodontal tissue. Toll-like receptors (TLRs) are prominent in the initial recognition of pathogens. Our previous study showed that TLR4 signaling is proinflammatory in periapical lesions induced by a polymicrobial endodontic infection. In contrast, the functional role of TLR2 in regulation of periapical tissue destruction is still not fully understood. Using TLR2 deficient (KO), TLR2/TLR4 double deficient (dKO), and wild-type (WT) mice, we demonstrate that TLR2 KO mice are highly responsive to polymicrobial infection-induced periapical lesion caused by over activation of TLR4 signal transduction pathway that resulted in elevation of NF-kB (nuclear factor kappa B) and proinflammatory cytokine production. The altered TLR4 signaling is caused by TLR2 deficiency-dependent elevation of CD14 (cluster of differentiation 14), which is a co-receptor of TLR4. Indeed, neutralization of CD14 strikingly suppresses TLR2 deficiency-dependent inflammation and tissue destruction in vitro and in vivo. Our findings suggest that a network of TLR2, TLR4, and CD14 is a key factor in regulation of polymicrobial dentoalveolar infection and subsequent tissue destruction. Anat Rec, 299:1281-1292, 2016. © 2016 Wiley Periodicals, Inc. |
Databáze: | OpenAIRE |
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