Study of H/D-exchange reaction kinetics of polypeptides
| Autor: | A. V. Chudinov, I. V. Sulimenkov, Alexander R. Pikhtelev, V. I. Kozlovskii |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Journal of Analytical Chemistry. 65:1517-1523 |
| ISSN: | 1608-3199 1061-9348 |
| Popis: | One of the possible methods for 3D protein structure investigation is the study of gas-phase hydrogen/deuterium (H/D) exchange reaction between protein ions and a D-containing reactant gas, e.g., D2O or ND3. A segmented radio frequency quadrupole (RFQ) was used as a molecule-ion reactor to study gasphase H/D-exchange of protonated ions of three different peptides. The ions were produced in an electrospray ion source. The RFQ is a part of ion transport interface of a high resolution orthogonal time-of-fiight mass spectrometer (O-TOF MS). The RFQ was modified for a linear ion trap (LIT) mode of operation to increase a dwell time of target ions inside the RFQ. Phase-sensitive operation of the LIT and the O-TOF MS was controlled by custom developed PC executive program. The reaction mixture of N2 and ND3 was injected into the reactor, while keeping its partial pressure in the range of 10−3–10−2 mbar, and corresponding ND3 concentration in the range of 1013–1014 cm−3. It was possible to vary the ion dwell time in the reactor between 30 ms and 1 s. H/D-exchange was studied for leucine enkephalin, gramicidin S and apamin. The data analysis based on statistical approach has shown a principal possibility to distinguish different mobile H-atoms of peptides, taking part in H/D-exchange, according to reaction rates. |
| Databáze: | OpenAIRE |
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