| Autor: |
Gek San Tan, Maxey C. M. Chung, Jason C. H. Neo, Siaw Ling Lo, Teck Keong Seow, Rosa C. M. Y. Liang |
| Rok vydání: |
2006 |
| Předmět: |
|
| Zdroj: |
Proteomics: Biomedical and Pharmaceutical Applications ISBN: 1402023227 |
| DOI: |
10.1007/1-4020-2323-5_7 |
| Popis: |
Proteome analysis of HCC is an emerging area of research in the discovery of novel proteins as early biomarkers for diagnosis and potential therapeutic targets for the treatment of HCC. Although numerous publications have appeared on the gene expression analysis of HCC by cDNA microarrays, there is still a need to analyze the differentially expressed proteins in the cellular proteome as many protein modifications are not reflected by changes at the RNA level. New and improved proteomic technologies such as DIGE, and ICAT labelling in combination with mass spectrometric analysis will drive and accelerate the next phase of expression profiling of HCC. There will also be a concerted effort in performing proteome analysis at the subcellular level (subproteomics) in order to detect and identify low abundance proteins which may be missed at the global expression profile of the whole cell. In addition the localization sites of these identified proteins will provide clues to their functions. Finally, it is hoped that integration of proteome-based approaches with data from genomic profiling will lead to a better understanding of hepatocarcinogenesis which will then contribute to the direct translation of the research findings into clinical practice (Selinger and Kellner, 2002). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
