Some aspects of xylose isomerase constitutive biosynthesis in Arthrobacter nicotianae

Autor: I. O. Tamkovich, L. I. Sapunova, A. G. Lobanok
Rok vydání: 2010
Předmět:
Zdroj: Applied Biochemistry and Microbiology. 46:400-404
ISSN: 1608-3024
0003-6838
Popis: The specific features of biosynthesis of the cell-bound xylose isomerase by the actinobacterium Arthrobacter nicotianae BIM V-5 were studied. It was demonstrated that the constitutive synthesis of this enzyme in the studied bacteria, not subject to catabolite repression, was inhibited by xylulose, an intermediate product of xylose utilization and the final product of its enzymatic isomerization. Short-term experiments demonstrated that xylulose at a concentration of 0.005% almost completely repressed the xylose isomerase synthesis in A. nicotianae. This effect was independent of the time moment when the repressor was added to the cultivation medium and was not associated with its influence on the catalytic activity of the enzyme.
Databáze: OpenAIRE