Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver
| Autor: | Tsuyoshi Shimamura, Kenji Wakayama, Satoru Todo, Daisuke Fukumori, Ikuroh Ohsawa, Shingo Shimada, Moto Fukai, Takahisa Ishikawa, Maki Shibata, Taichi Kimura, Kenichiro Yamashita, Akinobu Taketomi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Biomedical Engineering Ischemia Medicine (miscellaneous) Cold storage Bioengineering medicine.disease_cause Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Viaspan medicine.diagnostic_test Chemistry General Medicine Glutathione medicine.disease 030104 developmental biology Endocrinology Biochemistry 030220 oncology & carcinogenesis Liver function tests Reperfusion injury Perfusion Oxidative stress |
| Zdroj: | Artificial Organs. 40:1128-1136 |
| ISSN: | 0160-564X |
| Popis: | Hydrogen gas reduces ischemia and reperfusion injury (IRI) in the liver and other organs. However, the precise mechanism remains elusive. We investigated whether hydrogen gas ameliorated hepatic I/R injury after cold preservation. Rat liver was subjected to 48-h cold storage in University of Wisconsin solution. The graft was reperfused with oxygenated buffer with or without hydrogen at 37° for 90 min on an isolated perfusion apparatus, comprising the H2 (+) and H2 (-) groups, respectively. In the control group (CT), grafts were reperfused immediately without preservation. Graft function, injury, and circulatory status were assessed throughout the perfusion. Tissue samples at the end of perfusion were collected to determine histopathology, oxidative stress, and apoptosis. In the H2 (-) group, IRI was indicated by a higher aspartate aminotransferase (AST), alanine aminotransferase (ALT) leakage, portal resistance, 8-hydroxy-2-deoxyguanosine-positive cell rate, apoptotic index, and endothelial endothelin-1 expression, together with reduced bile production, oxygen consumption, and GSH/GSSG ratio (vs. CT). In the H2 (+) group, these harmful changes were significantly suppressed [vs. H2 (-)]. Hydrogen gas reduced hepatic reperfusion injury after prolonged cold preservation via the maintenance of portal flow, by protecting mitochondrial function during the early phase of reperfusion, and via the suppression of oxidative stress and inflammatory cascades thereafter. |
| Databáze: | OpenAIRE |
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