1H NMR Study of Heteroassociation of Ethidium Homodimer and Propidium Iodide in Water

Autor:	O. V. Rogova, Alexei N. Veselkov, S. A. Hernandez, D. A. Veselkov, David B. Davies, Maxim P. Evstigneev
Rok vydání:	2004
Předmět:	Phenanthridine Chemical shift Dimer Photochemistry Inorganic Chemistry chemistry.chemical_compound Crystallography chemistry Materials Chemistry Proton NMR Molecule Ethidium homodimer assay Propidium iodide Physical and Theoretical Chemistry Equilibrium constant
Zdroj:	Journal of Structural Chemistry. 45:793-799
ISSN:	1573-8779 0022-4766
DOI:	10.1007/s10947-005-0060-3
Popis:	1D and 2D 1H NMR spectroscopy (500 MHz) was used to study heteroassociation of the bifunctional intercalator ethidium homodimer (EBH) with a phenanthridine dye propidium iodide (PI) in an aqueous salt (0.1 mol/l NaCl). A physical model of equilibrium between various associated forms of ethidium homodimer and propidium in solution is suggested, the most probable forms being 1:1 and 1:2 complexes of PI with the EBH monomer and dimer. The chemical shifts of the EBH molecule in heterocomplexes, as well as the equilibrium constants and the thermodynamic parameters (enthalpy, entropy) of heteroassociations, were calculated from the concentration and temperature dependences of the proton chemical shifts. It is concluded that the propidium molecule is built (intercalated) into the EBH monomer and dimer to form 1:1 and 1:2 complexes mainly stabilized by dispersive and hydrophobic interactions.
Databáze:	OpenAIRE
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