Determination of Doxycycline Hyclate by Batch and Reverse Flow Injection Analysis Based on the Oxidative Coupling Reaction with 3-Methyl-2-benzothiazolinone Hydrazone hydrochloride (MBTH)

Autor:	Sadeem Subhi Abed, Omar T. Hussein
Rok vydání:	2016
Předmět:	General Computer Science General Mathematics General Physics and Astronomy General Chemistry Agricultural and Biological Sciences (miscellaneous) General Biochemistry Genetics and Molecular Biology
Zdroj:	Baghdad Science Journal. 13:0489
ISSN:	2411-7986 2078-8665
DOI:	10.21123/bsj.2016.13.2.2ncc.0489
Popis:	New, simple and sensitive batch and reverse FIA spectrophotometric methods for the determination of doxycycline hyclate in pure form and in pharmaceutical preparations were proposed. These methods based on oxidative coupling reaction between doxycycline hyclate and 3-methylbenzothiazolinone-2-hydrazone hydrochloride (MBTH) in the presence ammonium ceric sulfate in acidic medium, to form green water-soluble dye that is stable and has a maximum absorbance at 626 nm. A calibration graph shows that a Beer's law is obeyed over the concentration range of 1-80 and 0.5-110 ?g.mL-1 of DCH for the batch and rFIA respectively with detection limit of 0.325 ?g.mL-1 of DCH for r-FIA methods. All different chemicals and physical experimental parameters affecting the development and stability of the colored product were carefully studied. The proposed methods were successfully applied for the determination of DCH in pharmaceutical preparations.
Databáze:	OpenAIRE
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