Human Papillomavirus Infection and Transitional Cell Carcinoma of the Bladder
| Autor: | Antonio Lopez-Beltran, L. Vicioso-Recio, A.L. Escudero, J.C. Carrasco-Aznar |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Bladder cancer
Hybridization probe virus diseases Cell Biology In situ hybridization Biology medicine.disease Virology Molecular biology female genital diseases and pregnancy complications Koilocyte Pathology and Forensic Medicine Transitional cell carcinoma Antigen Carcinoma medicine Immunohistochemistry |
| Zdroj: | Pathology - Research and Practice. 192:154-159 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/s0344-0338(96)80210-x |
| Popis: | Summary The aim of the present research was to investigate human papillomavirus (HPV) infection by means of immunohistochemistry and in situ hybridization in 7 6 bladder cancer specimens. A biotinylated DNA probe that recognizes HPV 6/11, HPV 16/18 and HPV 31/33/35 was used for in situ hybridization. A polyclonal antibody recognizing HPV capsid antigen (HPVcAg) was used for immunohistochemistry. In situ hybridization and immunohistochemistry were developed by alkaline phosphatase and immunogold-silver techniques respectively. Our results showed that 25 (32.8%) out of 76 bladder carcinoma specimens reacted with HPVcAg. Twelve (15.7%) out of 76 cases were positive for HPV 16/18-DNA using non-isotopic in situ hybridization. Sixteen cases had koilocytosis. No positive signals were found for HPV 6/11 or 31/33/35-DNA probes. |
| Databáze: | OpenAIRE |
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