| Autor: |
Kevin W. Zhu, Shawn D. Burton, Maira H. Nagai, Justin D. Silverman, Claire A. de March, Matt Wachowiak, Hiroaki Matsunami |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Nature Communications. 13 |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-022-32267-3 |
| Popis: |
Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full text from SpringerLink