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G A A b st ra ct s patterns between normal colonic tissue (N), tubular adenoma (ADT), tubulovillous adenoma (ADTV) and colorectal cancer (CRC) biopsy tissue samples. Furthermore, our aim was to analyze and confirm the expression level of adenoma and CRC related miRNAs in matched plasma samples. METHODS: Sixty fresh frozen colorectal biopsy (n=20 N, n=11 ADT, n= 9 ADTV, n=20 CRC) and 16 matched plasma samples (n=4 N, n=4 ADT, n=4 ADTV, n=4 CRC) were collected and total RNA including miRNA was isolated (High Pure miRNA Isolation Kit, Roche,Germany). MiRNA expression analysis was performed on GeneChip® miRNA 3.0 Arrays (Affymetrix,USA). Then, the results of microarray analysis were confirmed on RT-qPCR arrays (microRNAReady-to-use PCRHuman Panel I+II V2.R; Exiqon, Denmark). The matched plasma samples were also evaluated by the same miRNA expression and RTqPCR arrays. The obtained results were analyzed by Expression Console (Affymetrix,USA). RESULTS: Out of the 1733 detectable number of miRNAs on the array, in normal biopsies 442, in adenomas 460, and in CRC 441 miRNA could be identified. In plasma these were 306, 334, and 321 miRNA, respectively. Interestingly, 12 miRNAs were upregulated (e.g. miR-31 logFC=3, p |
