COMPARISON BETWEEN BUFFY COAT SMEAR AND DAT FOR THE DIAGNOSIS OF VL IN SUDAN

Autor:	Rihab Mohamed Abdelrahman, Sayda Hassan El Safi
Rok vydání:	2019
Předmět:	Coat Pathology medicine.medical_specialty Visceral leishmaniasis business.industry parasitic diseases medicine Buffy coat Parasitological diagnosis medicine.disease business Peripheral blood
Zdroj:	World Journal of Biology and Biotechnology. 4:21
ISSN:	2522-6754 2522-6746
DOI:	10.33865/wjb.004.03.0225
Popis:	Visceral Leishmaniasis is considered by WHO as one of the leading diseases in the world and is present in 88 countries worldwide.It affects poor communities in peripheral areas. It infects the internal organs of the body. It is fatal if not treated.It is caused by the parasite L.donovani in the old world and L.infantum in the new world. Correct diagnosis is necessary if the disease is to be treated.Many diagnostic techniques are known but none is agreed upon worldwide.This study aims to compare two techniques for the diagnosis of VL in Sudan.37 patients was included in this study.Buffy coat smears were prepared from peripheral blood of these patients and were searched for the presence of the parasite.Serum from these blood samples was used to preform DAT technique.The sensitivity of buffy coat smear was 37.8% and that of DAT was 100%.When MacNemar test was conducted, it was found that DAT is better than buffy coat smear for the diagnosis of VL.We concluded that DAT is a better diagnostic technique and could be adapted for diagnosis of VL in Sudan.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::3a3fab8f7a68c4704151183d3cb6f1cd https://doi.org/10.33865/wjb.004.03.0225 Zobrazit plný text záznamu