Methods for Controlled Induction of Singular Rosette Cytoarchitecture Within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies
| Autor: | Randolph S. Ashton, Alireza Aghayee |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Morphogenesis Neural tube Biology Embryonic stem cell Neural stem cell Cell biology Rosette (zoology) 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Neural ensemble Cytoarchitecture medicine Induced pluripotent stem cell 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071611739 |
| DOI: | 10.1007/978-1-0716-1174-6_13 |
| Popis: | Neurally differentiating human pluripotent stem cells (hPSCs) possess the ability to self-organize into structures reminiscent of the developing fetal brain. In 2- and 3D cultures, this phenomenon initiates with formation of polarized areas of neural stem cells (NSCs), known as rosettes that resemble cross-sectional slices of the embryonic neural tube, i.e., the central nervous system (CNS) anlage. Thus, neural rosettes serve as an excellent starting point for bioengineering tissue models of all CNS tissues. Here, we provide detailed methods for bioengineering controlled induction of hPSC-derived neural assemblies with a biomimetic, singular neural rosette cytoarchitecture. |
| Databáze: | OpenAIRE |
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