LC with Column-Switching and Spectrophotometric Detection for Determination of Risperidone and 9-Hydroxyrisperidone in Human Serum
| Autor: | Manfred Gerlach, Friederike Vogel, Peter Riederer, Christoph Hiemke, Katrin M. Kirschbaum, Susann Finger, R. Burger |
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| Rok vydání: | 2008 |
| Předmět: |
Risperidone
Chromatography medicine.diagnostic_test Chemistry Organic Chemistry Clinical Biochemistry Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Blood serum Therapeutic drug monitoring medicine Sample preparation Solid phase extraction Acetonitrile Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Chromatographia. 67:321-324 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1365/s10337-007-0506-1 |
| Popis: | An automated high performance liquid chromatography with column-switching and ultraviolet detection was developed for the analysis of risperidone and 9-hydroxyrisperidone. The method needs minimum sample preparation and is useful for the detection down to a limit of 1 ng mL−1. Sample clean-up of serum was carried out on a CN 20 μm SPE-column using 8% (v/v) acetonitrile in water. Chromatographic separation was performed on ODS Hypersil C18 material with 38% (v/v) acetonitrile and 0.4% (v/v) TEMED in water. Application of the method to the analysis of serum samples confirmed its suitability for therapeutic drug monitoring of risperidone and 9-hydroxyrisperidone. |
| Databáze: | OpenAIRE |
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