Studying the Role of AMPK in Cardiac Hypertrophy and Protein Synthesis
| Autor: | Jean-Luc Balligand, Christophe Beauloye, Sandrine Horman, Florence Mailleux, Luc Bertrand |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cardiac function curve biology Chemistry AMPK NFAT 030204 cardiovascular system & hematology Phenformin EEF2 Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine AMP-activated protein kinase biology.protein Protein kinase A Transcription factor |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493975976 |
| DOI: | 10.1007/978-1-4939-7598-3_21 |
| Popis: | Pathological cardiac hypertrophy, which is a compensatory mechanism established to maintain cardiac function in response to neurohormonal or mechanical stresses, becomes maladaptive with time and frequently leads to heart failure. AMP-activated protein kinase (AMPK) has been extensively described in the literature to act as a break in cardiac hypertrophy development. Its anti-hypertrophic action mostly correlates with the inhibition of several important players of cardiac hypertrophy including protein synthesis and pro-hypertrophic gene expression pathways involving the transcription factor nuclear factor of activated T cells (NFAT) and the mitogen-activated protein kinases ERK1/2. In this chapter, we describe methodologies designed to evaluate cardiomyocyte hypertrophy and its major molecular mechanisms in response to AMPK activation. Two different compounds, AICAr and the biguanide phenformin, were used to promote AMPK activation. |
| Databáze: | OpenAIRE |
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