| Popis: |
The peptidoleukotriene antagonist, SK&F 107310 and its optical antipode, can be effectively separated on a chiral α-AGP column. Pre-treatment of the chiral α-AGP column with 0.1% v/v triethylamine in the mobile phase significantly improves the peak shape and detection sensitivity of the undesired enantiomer. Chromatography with the modifier in the mobile phase resulted in elution of the drug substance without retention at the solvent front. Without column pre-treatment, only a broad tailing peak was obtained for enantiomer 2, making low detection of it unattainable. |
