Polymerase Chain Reaction Used for Monitoring multiple Gene Integration in Agrobacterium ‐Mediated Transformation
| Autor: | Richard G. Stout, R. L. Ditterline, Nancy K. Blake |
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| Rok vydání: | 1991 |
| Předmět: |
Genetics
Rhizobiaceae Agrobacterium fungi food and beverages Agrobacterium tumefaciens biochemical phenomena metabolism and nutrition Biology biology.organism_classification Molecular biology law.invention Transformation (genetics) law Medicago sativa Agronomy and Crop Science Gene Polymerase chain reaction Southern blot |
| Zdroj: | Crop Science. 31:1686-1688 |
| ISSN: | 1435-0653 0011-183X |
| Popis: | Characterizing plants following Agrobacterium-mediated transformation has become an important consideration in the creation of transgenic plants. As an alternative to Southern blot analysis, polymerase chain reaction (PCR) was used to screen for the simultaneous integration of two foreign genes for neomycin phosphotransferase II (NPT II) and β-glucuronidase (GUS) in alfalfa (Medicago sativa L.) transformed with Agrobacterium tumefaciens |
| Databáze: | OpenAIRE |
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