In Situ Hybridization: Location of Plasma Protein Transcription

Autor:	Anthony J. Infante, Barbara H. Bowman, Jean B. Lum
Rok vydání:	1985
Předmět:	chemistry.chemical_classification Messenger RNA Cell In situ hybridization Biology Molecular biology Blood proteins medicine.anatomical_structure chemistry Transcription (biology) Transferrin Complementary DNA medicine Intracellular
DOI:	10.1016/b978-0-08-033215-4.50023-6
Popis:	Transcription of two human plasma proteins, haptoglobin (Hp) and transferrin (Tf) has been detected intracellularly in a variety of human cells and tissues. Sites of transcription are visualized as silver grains resulting from hybridization of radiolabelled Hp cDNA or Tf cDNA to the corresponding intracellular mRNA. This technique is effective in detecting transcription of plasma proteins, even in heterogeneous cell populations. It has facilitated the detection of Tf mRNA synthesis in a specific subset of human T-lymphocytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::39ca9c2dee6d753b16827471aaa73f57 https://doi.org/10.1016/b978-0-08-033215-4.50023-6 Zobrazit plný text záznamu