Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen
Autor: | Borgohain I, D. Borah, Probodh Borah, D. J. Dutta |
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Rok vydání: | 2018 |
Předmět: | |
Zdroj: | Indian Journal of Animal Research. |
ISSN: | 0976-0555 0367-6722 |
DOI: | 10.18805/ijar.b-3576 |
Popis: | The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility. |
Databáze: | OpenAIRE |
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