Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

Autor: Borgohain I, D. Borah, Probodh Borah, D. J. Dutta
Rok vydání: 2018
Předmět:
Zdroj: Indian Journal of Animal Research.
ISSN: 0976-0555
0367-6722
DOI: 10.18805/ijar.b-3576
Popis: The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.
Databáze: OpenAIRE